Product Name Actigel B Ultraflow 6 Product Code 2712-xx Bead size 40-160µm Agarose concentration 6% Spacer arm 7 atoms, hydrophilic Flow rate 4,000 cm/h Cleaning high salt, urea, guanidine, 50% ethanol, methanol Storage conditions 20% Ethanol, 2-8°C Functional group to attach Thiol, amino, or hydroxyl groups (pH dependent) Temperature stability range ambient Functional Ligand epibromohydrin Blocking agents Mercaptoethanol, ethanolamine Coupling in organic solvents yes Coupling in a packed column no Toxicity none pH stability range 2-7** Coupling conditions dependent on target functional group Chemical stability 8M Urea, 6M Guanidine, Ethanol, and Common Buffers Drug master file With U.S. Food & Drug Administration

Actigel B Ultraflow 6 is a reactive resin for covalent immobilization of peptides, carbohydrates, and other ligands containing amino, thiol, or hydroxyl groups. Coupling is pH-dependent: For coupling thiol groups: pH 7.5-8.5 (forms thioether linkage) For coupling amino groups: pH 8.5-11 (form stable 2° amine linkage) For coupling hydroxyl groups: pH 12 (forms ether linkage) Actigel B Ultraflow 6 features epibromohydrine functional groups that react with nucleophilic groups on the ligand. During coupling, the bromohydrine group is activated by adjusting pH for the desired linkage preference.

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