Product Name Actigel B Ultraflow 4 Product Code 2732-xx Bead size 40-160µm Agarose concentration 4% Spacer arm 7 atoms, hydrophilic Flow rate 4,500cm/h* Cleaning high salt, urea, guanidine, 50% ethanol, methanol Storage conditions 20% Ethanol, 2-8°C Functional group to attach Thiol, amino, or hydroxyl groups (pH dependent) Reactive group concentration QC, or test Temperature stability range ambient Functional Ligand epibromohydrin Blocking agents Mercaptoethanol, ethanolamine Coupling in organic solvents yes Coupling in a packed column no Toxicity none pH stability range 2-7** Coupling conditions dependent on target functional group Chemical stability 8M Urea, 6M Guanidine, Ethanol, and Common Buffers Drug master file With U.S. Food & Drug Administration

Actigel B Ultraflow 4 is a reactive resin for covalent immobilization of peptides, carbohydrates, and other ligands containing amino, thiol, or hydroxyl groups. Coupling is pH-dependent: For coupling thiol groups: pH 7.5-8.5 (forms thioether linkage) For coupling amino groups: pH 8.5-11 (form stable 2° amine linkage) For coupling hydroxyl groups: pH 12 (forms ether linkage) Actigel B Ultraflow 4 features epibromohydrine functional groups that react with nucleophilic groups on the ligand. During coupling, the bromohydrine group is activated by adjusting pH for the desired linkage preference.

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