| Product Name |
Q 9 Cellthru BigBead Plus |
| Product Code
|
11923-xx |
| Bead size |
800-1100µm |
| Matrix structure |
4% agarose, Hardened |
| Flow rate |
27,000cm/h* |
| Binding capacity |
25mg BSA/mL resin |
| Cleaning |
0.5M NaOH |
| Storage conditions |
20% Ethanol, 2-8°C |
| Temperature stability range
|
2°-121°C |
| Functional Ligand |
-N+(CH3)3 |
| Ion exchanger type |
Strong Anion |
| Counter ion |
Cl- |
| pH stability range |
2-14 |
| Chemical stability |
Common Aqueous, organic,
High Salt, denaturants |
| Physical stability |
Negligible volume changes
due to pH or Ionic state |
| Drug master file |
With U.S. Food & Drug
Administration |
|
Q 9 Cellthru BigBead Plus is a large particle, strong anion exchanger. This is the largest bead size range that we carry, and it is generally reserved for direct capture of high cell count feed streams. Ion exchange Cellthru BigBead Plus media is available in two particle size ranges (300-500µm and 800-1,100µm) with anion (DEAE/Q) or cation exchange (CM/SP) functionalities. The strong ion exchange (Q/SP) beads stay charged and maintain high capacities over a wide working pH range (pH 3-11). In weak ion exchangers (DEAE/CM), capacity is a function of pH.
Ion exchange Cellthru BigBead Plus resins are made of a hardened agarose matrix with ion exchange groups attached through a spacer arm. Hardening gives the matrix increased chemical and mechanical stability.
Cellthru BigBeads are used for more viscous feed streams and direct capture. In using the larger particle diameter beads to allow particulate material to flow through the column, you will likely need to retrofit the column with a larger porosity screen. We supply a 180µm screen with the beads for this purpose. People familiar with direct capture are likely accustomed to beads that do not function in a standard packed bed system, and associate direct capture with limitations on flow rate. In recognition of this short-coming of traditional direct capture, we have developed Cellthru BigBeads to function in standard packed-bed applications in short, fat columns (where the diameter of the column and the height are in a 1:1 ratio) with vertical scale-up potential using the same diameter. This makes pilot scale parameters more meaningful to production scale and reduces the time spent on method development.
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