| Product Name |
IDA Chelating Cellthru
BigBead |
| Product Code |
37311-xx |
| Bead size |
300-500µm |
| Agarose concentration |
4% |
| Molecular exclusion limit
|
20 million Daltons |
| Flow rate |
12,000cm/hr* |
| Binding capacity |
50mg Hemoglobin/ml resin
|
| Cleaning |
0.5M NaOH |
| Storage conditions |
20% Ethanol, 2-8°C |
| Temperature stability range
|
0-60°C |
| Functional Ligand |
Iminodiacetic acid |
| pH stability range |
2-14 |
| Chemical stability |
High Salt, 0.5M NaOH, Urea,
Guanidine, Organic Solvents: Ethanol (70%), Methanol (50%) |
| Drug master file |
With U.S. Food & Drug
Administration |
|
| IDA Chelating Cellthru BigBead is an immobilized
metal affinity chromatography (IMAC) resin that separates proteins
via their differences in affinity to various metal ions. There are
a number of metal-chelating resins that differ by how strongly they
bind a given metal. For instance, NTA resin is a tetra-dentate chelator,
leaving two coordination sites on a transition metal available to
bind complexing moieties on proteins, such as Histidine repeats. TED
resins are penta-dentate, leaving one less coordination site in the
chelated metal for binding proteins. Thus, TED typically has less
binding capacity than NTA. IDA Chelating resins are tri-dentate and
typically have very high binding capacities in comparison to other
chelators. IDA Chelating Cellthru BigBead is sent uncharged, for you
to decide which transition metal to complex with the resin. Typically,
users find Ni, Fe, Co, Cu, or Zn most effective. Optimizing the separation
usually requires some method development. IDA Chelating Cellthru BigBead
is recommended for viscous feed streams or direct capture in dilute
cell culture. |
|
