| Product Name |
Blue Cellthru BigBead |
| Product Code |
37312-xx |
| Bead size |
300-500µm |
| Agarose concentration |
4% |
| Molecular exclusion limit
|
20 million Daltons |
| Flow rate |
13,000 cm/h* |
| Binding capacity |
14mg HSA/mL resin |
| Cleaning |
0.1 M NaOH (<6 hours)
|
| Storage conditions |
20% Ethanol, 2-8°C |
| Reactive group concentration
|
7µmol/ml |
| Functional Ligand |
Cibacron Blue F3G-A |
| Leaching |
OD610 of supernatant =
6.9x10-4 after 105days |
| pH stability range |
2-13 |
| Chemical stability |
0.1M NaOH, high salt, urea,
guanidine, 0.1M Na2CO3 |
| Drug master file |
With U.S. Food & Drug
Administration |
|
| Blue Cellthru BigBead is a high-capacity dye-ligand
resin for direct purification of enzymes and proteins from crude,
or unclarified, feed streams such as serum, tissue extracts, cell
cultures and fermentation harvests. The large bead size allows particulate
material to flow between the beads while the soluble product is captured
by the dye functional group. The flow properties of the beads allow
the use of tall columns on scale-up. Blue Uniflow contains the blue
dye, Cibacron blue 3GA, which has a high affinity for several enzymes,
most notably the enzymes that bind a purine nucleotide. Some examples
of enzymes that bind to Cibacron Blue F3G-A are phosphofructokinase,
pyruvate kinase, and dehydrogenase. It is estimated that as many as
50% of enzymes are nucleotide-binding, so Blue-dyes are often used
as one of many purification steps in a process. They also bind to
several non-enzymatic proteins such as albumin, lipoproteins, and
blood coagulation factors. Blue Cellthru BigBead is extremely stable
in alkaline conditions and is compatible with most commonly used buffers.
Most Cibacron Blue chromatography resins will leach blue dye over
time, as is evidenced by the blue color of their storage buffer, reducing
their capacity without even using it. Sterogene’s linkage chemistry
ensures that this effect is drastically minimized, ensuring stable
binding capacities over the life of the resin and virtually eliminating
product contamination with leached dye. Whether capturing albumin
or purifying an enzyme, our stable linkage chemistry will provide
you with longer life spans, higher yields, and better cost effectiveness.
|
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