Product Name Activated ALD Cellthru BigBead Product Code 2711-xx Bead size 300-500µm Agarose concentration 4% Spacer arm 5 atoms, hydrophilic Flow rate 7400cm/hr* Cleaning 0.15 M NaOH** Storage conditions 20% Ethanol, 2-8°C Functional group to attach Primary Amines Reactive group concentration >40 µmoles monoaldehyde/ml Coupling efficiencies >90% Temperature stability range 2°-100°C (wet) Autoclavable Yes Functional Ligand Monoaldehyde Blocking agents ethanolamine Coupling in organic solvents yes Coupling in a packed column Yes Leaching <0.1 ppm Drug master file With U.S. Food & Drug Administration

Activated ALD Cellthru BigBead is an activated resin for immobilizing primary amines from small ligands to large proteins. Convenience and economy are the key advantages of Activated ALD Cellthru BigBead: coupling is simple, rapid and highly efficient. The larger particle size offers another advantage over traditional chromatography media by the increase interlumen space between the beads, allowing for a thicker, less clarified feed stock to be processed without impedence. During coupling, an instantaneous, reversible Schiff-base reaction takes place between the monoaldehyde groups and the primary amines of the ligand. The Schiff-base is then reduced to a stable secondary amine by the addition of a reducing agent so mild that it cannot reduce intramolecular disulfide bridges. ALD couples both acidic and basic proteins with the same high efficiency, typically >90% due to the high density of stable aldehyde groups. Coupling is highly reproducible. Hydrolysis or inactivation of the activated groups is the main cause of irreproducible coupling, frequently encountered with traditional coupling chemistries. Using ALD chemistry, coupling efficiency is simply a function of loading. No hydrolysis or inactivation of active groups takes place during the coupling reaction. This provides a highly reproducible coupling of ligands, critical for both research and manufacturing.

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