Product Name Superflow 6 Product Code 806-xx Bead size 60-160µm Agarose concentration 6% Molecular exclusion limit 6 million Daltons Flow rate 700cm/hr* Cleaning 1M NaOH Storage conditions 20% Ethanol, 2-8°C Temperature stability range 2°-100°C (wet) Autoclavable hydrated at pH 7 for 30 min pH stability range 2-14 Chemical stability 4M KI, 4M NaSCN, 8M urea, 6M guanidine HCl Physical stability Negligible volume changes due to changes in pH or ionic strength Drug master file U.S. Food & Drug Administration

Superflow 6 beads exhibit the same high biological stability as the Uniflow media. The 6% beads are further stabilized by a chemical hardening reaction that supports flow rates up to 5 times higher than the regular crosslinked 4% beads. Superflow 6 is an excellent choice as a support for immobilization or for gel filtration. The media are stable in organic solvents and aqueous solutions in the pH range 2-14. Oxidizing materials should be avoided since they can cause partial decomposition of the polysaccharide chains. The media are also resistant to high concentrations of chaotropic agents, such as 4M KI or 4M NaSCN. They can be sterilized by autoclaving at 121oC and pH 7 for 30min. Uniflow, Superflow and Ultraflow are provided as aqueous suspensions with 20% ethanol as preservative. They can be stored for years at 2-8oC. The media should not be frozen. For packing, prepare a 50% slurry and de-gas it if necessary. Packing can be performed by gravity or pump. Make sure that the operating flow rate does not exceed the packing flow rate. Equilibrate the column with 2-3 bed volumes of buffer. For elution, a peristaltic pump is recommended, as a consistent flow rate will aid in reproducible separations…The media can be cleaned in situ with one column volume of 0.5 M NaOH, which cleans and sanitizes the resin. Autoclaving, another sterilization method, can be done before packing the column. After cleaning with base, the column bed should be re-equilibrated with starting buffer. Superflow is an ideal support matrix for immobilizing biologically active substances. Superflow exhibits the very low nonspecific binding. This is most important when small amounts of biologics need to be isolated by affinity methods (e.g. diagnostic tests) or when a very high degree of purity is required (injectable therapeutics).

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