Product Name Uniflow 6 Product Code 706-xx Bead size 60-160µm Agarose concentration 6% Molecular exclusion limit 6 million Daltons Flow rate 700cm/hr* Cleaning 1M NaOH Storage conditions 20% Ethanol, 2-8°C Temperature stability range 2°-100°C (wet) Autoclavable hydrated at pH 7 for 30 min pH stability range 2-14 Chemical stability 4M KI, 4M NaSCN, 8M urea, 6M guanidine HCl Physical stability Negligible volume changes due to changes in pH or ionic strength Drug master file U.S. Food & Drug Administration

Uniflow 6 is an agarose-based gel filtration medium with bead-shaped particles, suitable for gel filtration and as an affinity chromatography support. The Uniflow line of products is available at three different agarose concentrations: 2%, 4%, and 6%. As agarose concentrations increase, matrix porosity decreases. Therefore the exclusion limit for globular proteins varies from 4,000kD (6% beads) to 40,000kD (2% beads). This allows the separation of a wide range of molecules including proteins, polysaccharides and nucleic acids. Uniflow 6 is a highly stable 6% agarose medium. Its agarose base features unique polysaccharide composition that resists biological degradation. Additional crosslinking greatly enhances the mechanical and chemical stability of the matrix and eliminates the problem of biodegradation.

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