Product Name Uniflow 4
Product Code 704-xx
Bead size 60-160µm
Agarose concentration 4%
Molecular exclusion limit 20 million Daltons
Flow rate 300cm/hr*
Cleaning 1M NaOH
Storage conditions 20% Ethanol, 2-8°C
Temperature stability range 2°-100°C (wet)
Autoclavable hydrated at pH 7 for 30 min
pH stability range 2-14
Chemical stability 4M KI, 4M NaSCN, 8M urea, 6M guanidine HCl
Physical stability Negligible volume changes due to changes in pH or ionic strength
Drug master file U.S. Food & Drug Administration
Uniflow 4 is an agarose-based gel filtration medium with bead-shaped particles, suitable for gel filtration and as an affinity chromatography support. Uniflow is available at three different agarose concentrations: 2%, 4%, and 6%.

As agarose concentrations increase, matrix porosity decreases. The exclusion limit for globular proteins varies from 4,000kD (6% beads) to 40,000kD (2% beads). This allows the separation of a wide range of molecules including proteins, polysaccharides and nucleic acids.

Uniflow is a highly stable medium. The matrix features a unique polysaccharide composition that resists biological degradation. Additional crosslinking greatly enhances the mechanical and chemical stability of the matrix and eliminates the problem of biodegradation.
 
back>>