Product Name Sulfated Hiflow Product Code 10133-xx Bead size fibers Matrix structure Hardened Cellulose Flow rate 1000cm/h* Binding capacity up to 35mg lysozyme/mL resin Cleaning 0.5M NaOH; 8M Urea; 6M Guanidine Hydochloride Storage conditions 20% Ethanol, 2-8°C Reactive group concentration 8µmol/ml Temperature stability range ambient Functional Ligand SO4 Ion exchanger type Strong cation Counter ion Na+ pH stability range 2-13 Chemical stability 0.5M NaOH; 8M Urea; 6M Guanidine Hydochloride; Ethanol, and Common Buffers Physical stability Negligible volume changes due to pH or Ionic state Drug master file With U.S. Food & Drug Administration

Sulfated Hiflow is a cellulose-based chromatography particle that mimics the affinity of heparin or dextran sulfate for many proteins: It can function as an affinity support for some plasma proteins, cellular growth factors and lipases. Traditionally, virus purification is carried out by filtration and centrifugation. However, the biohazards involved with the handling of many of the viruses limit the utility of open systems in scale up purification of viral materials. Meanwhile, progress in the development of vaccines and clinical diagnostics have created a demand for large volumes of highly purified and concentrated viruses and viral antigens. This has spurred demand for safer, simpler and more efficient virus purification methods, such as Sulfated Hiflow chromatographic resin. Viruses need to be purified from culture media containing host cells and other contaminants such as proteins, proteases and nucleases. Chromatographic purification on a closed column assures safety and product sterility and is more effective than ultracentrifugation at removing contaminants. Excessive product handling is avoided and safety concerns are reduced. The gentle binding and elution conditions associated with the technique provide high product yield, while simultaneous concentration and purification reduce processing steps, time and cost. Sulfated Hiflow resin was developed specifically for the rapid and effective purification and concentration of viruses and, viral/microbial antigens. It exhibits affinity for a wide range of viruses, primarily the enveloped type. Retroviruses are among those exhibiting the highest affinity for Sulfated Hiflow. Related viral or microbial antigens and heparin-binding proteins can also be purified easily. Sulfated Hiflow resin consists of a hardened cellulose matrix, treated to provide an optimal concentration of sulfate ester functionality on the 6-position of cellobiose. The density of sulfonate groups provides optimal affinity binding for a wide range of viruses and antigens. Sulfated Hiflow settles rapidly and has excellent resistance to generating fines. The fibrous medium resists compression, providing high flow for rapid processing, even in large columns. It is well-suited for both batch and column operations. Using Sulfated Hiflow can also reduce endotoxin from microbial sources because it has a very low affinity for endotoxin, an aliphatic molecule that is normally removed with affinity reagents such as ActiClean Etox.

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