| Product Name | DNA Etox |
| Product Code | 2706-xx |
| Bead size | 40-160µm |
| Agarose concentration | 4% |
| Flow rate | 2400cm/h* |
| Binding capacity | Protein binding: >500,000 EU/mL resin ; Plasmid endotoxin binding: >1,500 EU/mL resin (in plasmid prep) |
| Cleaning | 1M NaOH for 2-16 h |
| Storage conditions | 20% Ethanol, 2-8°C |
| Cycle times | 100 |
| Temperature stability range | 0-60°C |
| Functional Ligand | Nontoxic and nonmutagenic (proprietary) |
| Leaching | <0.1 ppm |
| Toxicity | none |
| pH stability range | 2-14 |
| Chemical stability | 1M NaOH, 8M Urea, 6M Guanidine, Ethanol, and Common Buffers, incompatible with heavy metals |
| Drug master file | With U.S. Food & Drug Administration |
| Removing endotoxin from DNA is often difficult
because of the similarity in the physicochemical characteristics of
DNA and endotoxin. The most commonly used methods—ultrafiltration,
size exclusion and ion exchange chromatography—have limited
utility in this area. Consequently, endotoxin removal is typically
tedious and often incurs significant losses of DNA during the process.
DNA Etox overcomes these difficulties. DNA Etox is a high efficiency, solid-phase reagent with a high affinity for endotoxin. The affinity of the immobilized ligand for DNA is very low, delivering a purified mass yield typically exceeding 90%. DNA Etox utilizes Sterogene's proprietary ALD coupling chemistry (see Actigel ALD for more), an immobilization technique that produces a highly stable secondary amine linkage. Leaching is negligible (less than 0.1ppm, the sensitivity limit of the assay). DNA Etox can be used either in a batch mode or in a standard chromatography column. For batches, the settled gel is simply added directly to the DNA solution and vortexed. Several contact times ranging from 3 to 20 min should be tested to establish the most complete removal of endotoxin. The immobilized ligand is nontoxic, nonmutagenic and biodegradable. DNA Etox can be readily regenerated with 1M NaOH and reused. This is made possible by the stability of the ligand and the matrix, as well as the ALD linkage chemistry. DNA Etox can also be sterilized by autoclaving. |
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