Product Name Actigel ALD
Product Code 2701-xx
Bead size 60-160 µm
Agarose concentration 4%
Spacer arm 5 atoms, hydrophilic
Flow rate 300cm/h*
Binding capacity Up to 60 mg/ml
Cleaning 0.15 M NaOH**
Storage conditions 20% Ethanol, 2-8°C
Functional group to attach Primary Amines
Reactive group concentration 60-78 µmoles/ml
Coupling efficiencies >90%
Temperature stability range 2°-100°C (wet)
Autoclavable Yes
Functional Ligand Monoaldehyde
Blocking agents Mercaptoethanol, ethanolamine***
Coupling in organic solvents yes
Coupling in a packed column Yes
Leaching <0.1 ppm
pH stability range 2-13
Coupling conditions pH 3-10, 0-40oC, 2-12hours
Chemical stability 0.1M NaOH, 8M Urea, 6M Guanidine, Ethanol, and Common Buffers
Drug master file With U.S. Food & Drug Administration
Actigel ALD is an activated resin for immobilizing primary amines from small ligands to large proteins. Convenience and economy are the key advantages of Actigel ALD: coupling is simple, rapid and highly efficient.

During coupling, an instantaneous, reversible Schiff-base reaction takes place between the monoaldehyde groups and the primary amines of the ligand. The Schiff-base is then reduced to a stable secondary amine by the addition of a reducing agent so mild that it cannot reduce intramolecular disulfide bridges. ALD couples both acidic and basic proteins with the same high efficiency, typically >90% due to the high density of stable aldehyde groups.

Coupling is highly reproducible. Hydrolysis or inactivation of the activated groups is the main cause of irreproducible coupling, frequently encountered with traditional coupling chemistries.

Using ALD chemistry, coupling efficiency is simply a function of loading. No hydrolysis or inactivation of active groups takes place during the coupling reaction. This provides a highly reproducible coupling of ligands, critical for both research and manufacturing.
 
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