| Product Name |
IDA Chelating Ultraflow
4 |
| Product Code |
37411-xx |
| Bead size |
40-160µm |
| Agarose concentration |
4% |
| Flow rate |
1,500cm/hr* |
| Binding capacity |
60mg Hemoglobin/ml resin?
|
| Cleaning |
0.5M NaOH |
| Storage conditions |
20% Ethanol, 2-8°C |
| Reactive group concentration
|
acid base titration |
| Temperature stability range
|
0-60°C |
| Functional Ligand |
Iminodiacetic acid |
| pH stability range |
2-14 |
| Chemical stability |
High Salt, 0.5M NaOH, Urea,
Guanidine, Organic Solvents: Ethanol (70%), Methanol (50%) |
| Drug master file |
With U.S. Food & Drug
Administration |
|
| IDA Chelating Ultraflow 4 is an immobilized metal
affinity chromatography (IMAC) resin that separates proteins via their
differences in affinity to various metal ions. There are a number
of metal-chelating resins that differ by how strongly they bind a
given metal. For instance, NTA resin is a tetra-dentate chelator,
leaving two coordination sites on a transition metal available to
bind complexing moieties on proteins, such as Histidine repeats. TED
resins are penta-dentate, leaving one less coordination site in the
chelated metal for binding proteins. Thus, TED typically has less
binding capacity than NTA. IDA Chelating resins are tri-dentate and
typically have very high binding capacities in comparison to other
chelators. IDA Chelating Ultraflow 4 is sent uncharged, for you to
decide which transition metal to complex with the resin. Typically,
users find Ni, Fe, Co, Cu, or Zn most effective. Optimizing the separation
usually requires some method development. IDA Chelating Ultraflow
4 is recommended for clarified feed streams. |
|
