Immobilize your own antigen or peptide on our
proprietary Actigel ALD. Then Isolate your antibody quickly and effortlessly
with your custom affinity resin. Afterwards, use the columns provided
in the kit to do buffer exchange and concentrate the purified antibody
in about an hour.
Features:
QUICKPure utilizes a single immunoaffinity purification procedure
to isolate specific antibody from anti-serum, in about an hour. This
system requires no dialysis or external concentration steps, and no
individual method development. QUICKPure is configured so that after
antigen immobilization, any specific antibody can be purified under
non-denaturing conditions. Importantly, this system limits the amount
of nonspecific serum antibodies common to purifications using conventional
absorbents (e.g. Protein A, Protein G). QUICKPure is fast and economical,
including all the prepacked columns and buffers necessary for 25 purifications.
The kit includes three columns. The first column is an activated resin
to which you will immobilize your antigen, and then use as an affinity
capture column. The flowthru is collected in a waste beaker. Then
the second and third columns are fitted via lure locks to the bottom
of the capture column for the elution step. The eluted antibody is
placed in to an appropriate buffer in the second column, thereby eliminating
the time consuming step of dialysis. Then the antibody is concentrated
in the third column, allowing for the recovery of antibodies from
relatively low titer expression systems. |
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