Product Name QUICKMab Systems Human Kappa Product Code QSH02K Package 25 runs / kit

Kit Contains: 5ml capture column 50ml buffer exchange column 1.5ml concentration column All buffers required for 25 runs All tubing, connectors, and instructions neccessary for operation ( A QUICKBuffer Replacement Kit is available for purchase for an additional 25 runs) Isolate your human antibody quickly and effortlessly from other IgGs with immobilized human kappa chain affinity resin. Then use the columns provided in the kit to do buffer exchange and concentrate the purified antibody in about an hour. Features: QUICKMab Systems Human Kappa utilizes a single immunoaffinity purification procedure to isolate human antibodies from anti-serum. This system eliminates the need for dialysis, external concentration steps, and individual method development. QUICKMab is configured so that any human antibody with a kappa chain can be purified under non-denaturing conditions. Moreover, this system severely limits the amount of nonspecific serum antibody binding common to purifications using conventional absorbents (e.g. Protein A, Protein G). QUICKMab is fast and economical, including all the prepacked columns and connections with buffers necessary for 25 purifications. The kit includes three columns. The first column is the capture column, which binds the human (QSH02K) IgGs selectively. The flowthru is collected in a waste beaker. Then the second and third columns are fitted via lure locks to the bottom of the capture column for the elution step. The eluted antibody is placed in to an appropriate buffer in the second column, thereby eliminating the time consuming step of dialysis. Then the antibody is concentrated in the third column, allowing for the recovery of antibodies from relatively low titer expression systems.

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