| Product Name |
Pepsin Actigel |
| Product Code |
6701-xx |
| Bead size |
60-160µm |
| Agarose concentration |
4% |
| Spacer arm |
5 atoms |
| Flow rate |
500cm/h* |
| Cleaning |
20mM sodium acetate, pH
4.5 |
| Storage conditions |
0.1M Sodium Acetate, 0.05%
Sodium Azide, 50% Glycerol, pH 4.5, 2-8°C |
| Reactive group concentration
|
?3mg/ml |
| Functional Ligand |
Pepsin enzyme |
| Leaching |
<0.1 ppm |
| pH stability range |
2-4 |
| Chemical stability |
common buffers at pH optima
|
| Drug master file |
With U.S. Food & Drug
Administration |
| Product Name |
CAP-8 IgG Purification
Kit |
| Product Code |
C8100-01 |
| Package |
kit |
|
Pepsin Actigel is made by immobilizing high-grade
pepsin onto Sterogene’s Actigel ALD. This ensures
an exclusively uniform, stable secondary amine linkage between the
reactive monoaldehyde groups of the activated resin and the primary
amino groups of the pepsin. Coupling efficiency is simply a function
of loading, so higher pepsin concentrations are available upon request
should they be desired.
IgG class antibodies are known to exhibit different sensitivities
to pepsin cleavage. IgG1 and IgG2a antibodies are relatively resistant
to pepsin by nature. However, IgG2b, IgG3, and IgG4 do not exhibit
such a resistance. IgG antibodies can be made more susceptible to
pepsin cleavage with a short preliminary incubation at pH 2.8. Brief
digestion with pepsin is sufficient for full conversion into F(ab)2
fragments afterwards. Alternatively, a longer incubation for 18-24
hours at 37oC, or a brief incubation at an elevated temperature (50min
at 70oC) can be used to achieve complete digestion of IgG. |
|
