Product Name Papain Actigel Product Code 6700-xx Bead size 60-160µm Agarose concentration 4% Spacer arm 5 atoms Flow rate 1500cm/h* Cleaning 20mM Sodium Phosphate, pH 6.2, 1mM DTT, 5mM EDTA Storage conditions 0.1M Sodium Acetate, 0.05% Sodium Azide, 50% Glycerol, pH 4.5, 2-8°C Reactive group concentration ?20mg/ml Functional Ligand Papain enzyme Leaching <0.1 ppm pH stability range 4-7 Chemical stability common buffers at pH optima Drug master file With U.S. Food & Drug Administration Product Name CAP-8 IgG Purification Kit Product Code C8100-01 Package kit

Papain Actigel is made by immobilizing high-grade papain onto Sterogene’s Actigel ALD. This ensures an exclusively uniform, stable secondary amine linkage between the reactive monoaldehyde groups of the activated resin and the primary amino groups of the pepsin. Coupling efficiency is simply a function of loading, so higher papain concentrations are available upon request should they be desired. Papain is a thiol protease and requires a mild reducing environment for activity. Thus cleavage is carried out in the presence of thiol compounds, such as cysteine or mercaptoethanol. Cleavage occurs in the hinge region of the IgG molecule, yielding Fab fragments and Fc fragments. The F(ab) fragment is monovalent, and therefore cannot stimulate antigen crosslinking or precipitation. Sterogene’s Protein A Ultraflow 4 can be used to scavenge the Fc fragments and any undigested IgG molecules.

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