Product Name CAP-8 IgG Purification Kit Product Code C8100-01 Package kit

Contains: 1L CAP-8 precipitating solution 9mL SP Thruput Plus prepacked column, tubing, connectors, and instructions. Features: Optimized Caprylic Acid Precipitation (CAP-8 Precipitating Solution) Precipitates most non-IgG proteins Removes lipids Inactivates>106 enveloped viruses Conditions yield a precipitate that is easy to remove by filtration alone No clarification of feed stream needed Cation Exchange (SP Thruput Plus) Purifies the IgG from the supernatant Supernatant of the precipitate contains approximately 80-85% IgG Purified to ~95% purity using cation exchange chromatography Our CAP-8 IgG Purification Kit combines two conventional, non-affinity purification methods to obtain IgG purity. The total IgG yield from this kit is approximately 70%. The new optimized precipitation conditions yield a granular precipitate that is easy to remove by filtration alone. This simplifies scale-up considerably. In addition, there is no need for clarification of the starting serum, ascites, or egg yolk prior to purification since the precipitation step also removes lipids and hydrophobic proteins. From the filtrate, IgG is then purified on the SP Thruput Plus column. The IgG is obtained with both high yield and purity. This method was developed for IgG purification from serum, ascites and egg yolk. The procedure delivers high yield, is reproducible and easy to scale up. High-purity IgG is obtained in a one-step cation exchange chromatography. Additional benefits are the delipidating and viricidal effects of caprylic acid which simplify sample preparation and facilitate process validation. The method has comparable performance to affinity purifications at the fraction of the cost.

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